Generator

Part:BBa_K737005

Designed by: Li Kang   Group: iGEM12_OUC-China   (2012-09-18)

Inducible GVPC Generator

This part contains a constitutive promoter (J23106), a coding region of TetR protein, a terminator (B0015), a promoter repressed by TetR protein (R0040) a coding region of GvpC and GFP and a terminator (B0015) behind it. This part can be used to induce the strength of promoter (R0040) by Tet and indicate the content of GvpC by fluorescence of GFP. In short, it can regulate the expression of GvpC. We can use different concentration of Tet to regulate the strength of promoter (R0040) ahead GvpC Protein and GFP. And We can know the approximate concentration of GvpC by the fluorescence unit of GFP. To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2148


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Categories
Parameters
None